Invitrogen™ BRCA1 Monoclonal Antibody (8F7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA123162

Catalog No. PIMA123162

Description

Recommended positive controls: 293T. For immunohistochemistry (paraffin), perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1M citrate buffer at 95ºC in water bath for 20 minutes). Avoid nonspecific binding by incubation in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes.

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Specifications

• Antigen: BRCA1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P38398, P48754
• Gene Symbols: BRCA1
• Immunogen: Protein fragment expressed in E. coli corresponding to amino acids 341-748.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: 8F7
• Conjugate: Unconjugated
• Gene: BRCA1
• Gene Alias: brca 1; Brca1; brca-1; BRCA1, DNA repair associated; BRCA1/BRCA2-containing complex, subunit 1; BRCAI; BRCC1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein 1; breast cancer 1; breast cancer 1 early onset; breast cancer 1, early onset; breast cancer associated 1; breast cancer type 1 susceptibility protein; Breast cancer type 1 susceptibility protein homolog; BROVCA1; BROVCA12; Delta772-3095; early onset breast cancer 1; Fanconi anemia, complementation group S; FANCS; IRIS; OTTHUMP00000212147; OTTHUMP00000212148; OTTHUMP00000212149; OTTHUMP00000212150; OTTHUMP00000212151; OTTHUMP00000212155; PNCA4; PPP1R53; protein phosphatase 1, regulatory subunit 53; PSCP; RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1; RNF53; truncated BRCA1; truncated breast and ovarian cancer susceptibility protein 1; truncated breast and ovarian cancer sususceptibility protein 1; truncated breast cancer type 1 susceptibility protein
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 12189, 672
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid