Invitrogen™ BRD4 Chimeric Recombinant Rabbit Monoclonal Antibody (RAB-C131)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA548328

Catalog No. PIMA548328

Description

Specificity: This antibody recognizes BRD4 (Bromodomain-containing protein 4). It binds to a folded domain, amino acids 333-460. BRD4 is a chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory.

BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.

Specifications

• Antigen: BRD4 Chimeric
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% ProClin 300
• Gene Accession No.: O60885
• Gene Symbols: BRD4
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing BRD4 protein under non-denaturing conditions.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG κ

• Applications: ChIP Assay, ELISA, Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: RAB-C131
• Conjugate: Unconjugated
• Gene: BRD4
• Gene Alias: Brd4; BRD4-NUT FUSION; Brd5; bromodomain containing 4; bromodomain-containing 4; bromodomain-containing 5; bromodomain-containing protein 4; bromodomain-containing protein 4; LOW QUALITY PROTEIN: bromodomain-containing protein 4; CAP; chromosome-associated protein; fsh-like protein; HUNK1; HUNKI; Mcap; mitotic chromosome-associated protein; Protein HUNK1; WI-11513
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 23476
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid