Invitrogen™ BRD4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA585662

Catalog No. PIPA585662

Description

Keep as concentrated solution. Predicted reactivity: Pig (98%). Positive Control: 293T nuclear extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.

Specifications

• Antigen: BRD4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BRD4
• Gene Alias: Brd4; BRD4-NUT FUSION; Brd5; bromodomain containing 4; bromodomain-containing 4; bromodomain-containing 5; bromodomain-containing protein 4; bromodomain-containing protein 4; LOW QUALITY PROTEIN: bromodomain-containing protein 4; CAP; chromosome-associated protein; fsh-like protein; HUNK1; HUNKI; Mcap; mitotic chromosome-associated protein; Protein HUNK1; WI-11513
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23476, 57261
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.48 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: O60885, Q9ESU6
• Gene Symbols: BRD4
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human BRD4 . The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG