Invitrogen™ BRD4 Recombinant Rabbit Monoclonal Antibody (7L7M8)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA542659

Catalog No. PIMA542659

Description

Positive test controls include: HeLa, Jurkat. The target is usually found in the following locations: Chromosome, Nucleus. Immunogen sequence: IIKTPMDMGT IKKRLENNYY WNAQECIQDF NTMFTNCYIY NKPGDDIVLM AEALEKLFLQ KINELPTEET EIMIVQAKGR GRGRKETGTA KPGVSTVPNT T.

BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.

Specifications

• Antigen: BRD4
• Classification: Recombinant Monoclonal
• Concentration: 0.55 mg/mL
• Formulation: PBS with 0.05% BSA, 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O60885
• Gene Symbols: BRD4
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human BRD4 (O60885).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Clone: 7L7M8
• Conjugate: Unconjugated
• Gene: BRD4
• Gene Alias: Brd4; BRD4-NUT FUSION; Brd5; bromodomain containing 4; bromodomain-containing 4; bromodomain-containing 5; bromodomain-containing protein 4; bromodomain-containing protein 4; LOW QUALITY PROTEIN: bromodomain-containing protein 4; CAP; chromosome-associated protein; fsh-like protein; HUNK1; HUNKI; Mcap; mitotic chromosome-associated protein; Protein HUNK1; WI-11513
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23476
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid