Invitrogen™ BrdU Monoclonal Antibody (A9C7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA550620

Catalog No. FEMA550620

Description

Positive control: BrdU treated mouse embryo tissue, BrdU treated Hela, BrdU treated NIH/3T3, EdU treated mouse embryo tissue, EdU treated Hela, EdU treated NIH/3T3. Subcellular Location: Nucleus.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

Specifications

• Antigen: BrdU
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
• Clone: A9C7
• Conjugate: Unconjugated
• Gene Alias: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
• Immunogen: BrdU-OVA.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Mouse