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Invitrogen™ BrdU Monoclonal Antibody (BU20A)
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Catalog No. PIMA181890
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200 μg
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Catalog No. Quantity
PIMA181890 200 μg
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Catalog No. PIMA181890 Supplier Invitrogen™ Supplier No. MA181890
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Description

Mouse Monoclonal Antibody

For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd).

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
TRUSTED_SUSTAINABILITY

Specifications

Antigen BrdU
Applications Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone BU20A
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.4
Gene Alias 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Host Species Mouse
Immunogen Bromodeoxyuridine conjugated to BSA.
Purification Method Protein A
Quantity 200 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG1
Target Molecule Chemical
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