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Invitrogen™ BrdU Monoclonal Antibody (MoBU-1)
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Catalog No. 01675977
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100 μg
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Catalog No. Quantity
01-675-977 100 μg
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Catalog No. 01-675-977 Supplier Invitrogen™ Supplier No. MA119213
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Description

Mouse Monoclonal Antibody

This antibody reacts also specifically with 5-bromouridine (BrU), and it does not cross-react with EdU.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
TRUSTED_SUSTAINABILITY

Specifications

Antigen BrdU
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone MoBU-1
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 15 mM sodium azide; pH 7.4
Gene Alias 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Host Species Mouse
Immunogen BrdU conjugated to hemocyanine.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage 4°C, do not freeze
Product Type Antibody
Form Liquid
Isotype IgG1
Target Molecule Chemical
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