Invitrogen™ eBioscience™ BrdU Staining Kit for Flow Cytometry APC

Catalog No.	Quantity
50-112-5095	100 tests

Catalog No. 50-112-5095 Supplier Invitrogen™ Supplier No. 8817660042

Description

Includes

• BrdU
• DNase I
• Anti-BrdU APC Antibody (clone BU20A)
• BrdU Staining Buffer Concentrate (4X)
• Fixation/Permeabilization Diluent

Contains the necessary reagents and buffers for identifying and examining proliferating cells of mammalian species by flow cytometric analysis. This kit is optimized to achieve brighter staining compared to traditional protocols.

• Cycling cells are incubated with 5-bromo-2′-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis
• Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest

Specifications

• Detection Method: Fluorescence
• For Use With (Application): BrdU Staining
• For Use With (Equipment): Flow Cytometer
• Product Type: Staining Kit
• Format: Kit
• Product Line: eBioscience
• Quantity: 100 tests

Frequently Asked Questions (FAQs)

Can I also stain for intracellular targets such as transcription factors and cytokines using the eBioscience BrdU staining kit?

Yes, primary antibodies against additional intracellular targets can be added alongside the Anti-BrdU antibody. However, it is critical that you empirically determine whether these additional antibodies are compatible with this staining protocol.

Is the BrdU in the eBioscience BrdU staining kit supplied as a sterile formulation for in vivo use?

Yes, the BrdU is supplied as a sterile liquid. The vial should be opened under sterile conditions if it will be used in vivo.

Can I keep the diluted (1 mM) BrdU in the eBioscience BrdU staining kit at 4 degrees C? Can I refreeze it after use?

The stock (32.5 mM) BrdU can be frozen and thawed up to three times. Furthermore, the stock BrdU can be kept at 4 degrees C for up to one week. However, the diluted (1 mM) BrdU should be used immediately.

Have the BrdU Staining kits been tested on proliferating cells of different species?

We have used this kit to detect proliferating human and mouse cells. Other species have yet to be tested but feline, non-human primate and canine should also work.

What are the stopping points during flow cytometric analysis when using a BrdU staining kit?

Samples can be stored at 4 degrees C for up to 16 hours (i.e., overnight) after fixation with BrdU Staining Buffer (with or without washing in Flow Cytometry Staining Buffer) with no adverse effect on performance.

Can I refreeze the DNase I in BrdU Staining kits?

Yes, as supplied, the DNase I can be frozen and thawed up to three times.

How long can I keep the diluted DNase I in BrdU Staining kits at 4 degrees C?

Once diluted to a working concentration, the DNase I can be kept at 4 degrees C for up to 1 week. Longer storage under these conditions will result in a reduction in positive staining.

For Research Use Only. Not for use in diagnostic procedures.