BRF1 Polyclonal Antibody, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc IHC00270T

Catalog No. 50-157-1798

Description

The recommended shelf life for this product is 1 year from date of receipt. Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Orangutan, Monkey, Gorilla, Chimpanzee, Northern white-cheeked gibbon and Chinese ham

RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in maligt cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of maligt cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.

Specifications

• Antigen: BRF1
• Classification: Polyclonal
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene Accession No.: Q92994
• Gene Symbols: BRF1
• Immunogen: Between 627 and 677
• Quantity: 10 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry
• Conjugate: Unconjugated
• Gene: BRF1
• Gene Alias: Transcription factor IIIB 90 kDa subunit, TFIIIB90, hTFIIIB90, B-related factor 1, BRF-1, hBRF, TAF3B2, TATA box-binding protein-associated factor, RNA polymerase III, subunit 2, BRF1, BRF, GTF3B, TAF3B2, TAF3C
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2972
• Content And Storage: 4° C
• Form: Liquid