Invitrogen™ Bub1 Polyclonal Antibody

Catalog No.	Quantity
PIPA585234	100 μL

Catalog No. PIPA585234 Supplier Invitrogen™ Supplier No. PA585234

Description

Rabbit Polyclonal Antibody

Keep as concentrated solution. Predicted reactivity: Bovine (80%). Positive Control: 293T, A431, H1299, HepG2, Molt-4, Raji. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The BUB1 gene encodes a serine/threonine-protein kinase that is integral to the spindle assembly checkpoint, a crucial mechanism ensuring proper chromosome alignment and segregation during mitosis. BUB1 is involved in detecting and correcting errors in kinetochore-microtubule attachments, which is vital for maintaining genomic stability. It acts by delaying the onset of anaphase until all chromosomes are correctly attached to the spindle apparatus. BUB1 is recruited to kinetochores in early mitosis, where it phosphorylates several substrates to activate other checkpoint proteins, such as BUBR1 and MAD2, thereby preventing premature progression through mitosis. Mutations or aberrations in the expression levels of BUB1 are often associated with aneuploidy and chromosomal instability, contributing to cancer progression. BUB1 is thus considered a potential target for cancer therapeutics, aiming to exploit its role in the mitotic checkpoint to selectively eliminate tumor cells through checkpoint bypass or mitotic arrest.

Specifications

• Antigen: Bub1
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.63 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene: BUB1
• Gene Accession No.: O43683
• Gene Alias: Aamp; Aamp-rs; AL022991; angio associated migratory cell protein; angio-associated migratory cell protein; angio-associated migratory protein; angio-associated, migratory cell protein; AU040907; BUB1; BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1 mitotic checkpoint serine/threonine kinase; BUB1, mitotic checkpoint serine/threonine kinase; BUB1A; BUB1L; budding uninhibited by benzimidazoles 1 homolog; budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae); C80208; D2Xrf87; hBUB1; mBUB1; mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase
• Gene Symbols: BUB1
• Host Species: Rabbit
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human BUB1. The exact sequence is proprietary.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 699
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG