BUBR1 KinaseMouse anti-Human, Clone: 8G1, Rockland™

Catalog No.	Quantity
50-199-0542	100 μg

Catalog No. 50-199-0542 Supplier Rockland Immunochemicals Supplier No. 200301902

Description

Mouse Monoclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Directed against human BUBR1 Kinase protein. The product was purified from tissue culture supernatant by chromatography. This antibody has only been tested on human cells. Reactivity against homologues from other sources is not known.

BUBR1 is a member of the Ser/Thr protein kinase family. It is a component of the mitotic checkpoint that delays anaphase until all chromosome are properly attached to the mitotic spindle. Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.

Specifications

• Antigen: BUBR1 Kinase
• Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 8G1
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: BUB1B
• Gene Accession No.: O60566
• Gene Alias: AU045529; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; BUB1 mitotic checkpoint serine/threonine kinase B; Bub1A; BUB1B; BUB1B, mitotic checkpoint serine/threonine kinase; BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 beta; budding uninhibited by benzimidazoles 1 homolog beta; budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae); hBub1; hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; Protein SSK1; SSK1
• Gene Symbols: BUB1B
• Host Species: Mouse
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 701
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1