Invitrogen™ BUBR1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143857

Catalog No. PIPA5143857

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human Hek293 whole cell, human K562 whole cell, human HL-60 whole cell. IHC: mouse testis tissue, rat testis tissue, human colon cancer tissue, human mammary cancer tissue, mouse testis tissue, rat testis tissue, human colon cancer tissue, human mammary cancer tissue. ICC/IF: U20S cell. Flow: U87 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

BUBR1 is a member of the Ser/Thr protein kinase family. It is a component of the mitotic checkpoint that delays anaphase until all chromosome are properly attached to the mitotic spindle. Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.

Specifications

• Antigen: BUBR1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BUB1B
• Gene Alias: AU045529; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; BUB1 mitotic checkpoint serine/threonine kinase B; Bub1A; Bub1b; BUB1B, mitotic checkpoint serine/threonine kinase; BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 beta; budding uninhibited by benzimidazoles 1 homolog beta; budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae); hBub1; hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint kinase MAD3L; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; Protein SSK1; SSK1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12236, 171576, 701
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene Accession No.: O60566, Q9Z1S0
• Gene Symbols: BUB1B
• Immunogen: E.coli-derived human BubR1/BUB1B recombinant protein (Position: K26-E448).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG