BV480 CD103 Rat anti-Mouse, Brilliant Violet 480, Clone: M290, BD

Catalog No.	Quantity
BDB566201	25 μg

Catalog No. BDB566201 Supplier BD Biosciences Supplier No. 566201

Description

Rat Monoclonal Antibody

The M290 antibody specifically binds to CD103, the chain of IEL 7 integrin. CD103 has a unique and fairly restricted tissue distribution. It is expressed on almost all intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells (DEC), subpopulations of peripheral T cells, and distinct subsets of fetal, neonatal, and adult thymocytes. E-cadherin is the epithelial cell ligand for α IEL β 7 integrin. The ordered expression of α IEL during thymocyte development (which occurs under the influence of the thymic epithelium), high level of α IEL expression on peripheral T cells in epithelial tissues (IEL and DEC), and expression of CD103 on a subset of CD8+ lymphocytes responding to allogeneic epithelial cells, suggest that α IEL β 7 integrin may have a common role in the interactions of T lymphocytes with epithelia during T-cell maturation and effector functions. CD103 is thought to play a role in allograft rejection. The M290 antibody is reported to efficiently inhibit α IEL β 7-mediated adhesion in in vitro /assays.

The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436nm and Em Max at 478nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.
BD Horizon™ BV480 is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436nm and Em Max at 478nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon™ BV510 (525/40nm) filter set. BV480 has less spillover into the BD Horizon™ BV605 detector and, in general, is brighter than BD Horizon BV510.  Due to similar excitation and emission properties, BV480, BV510 and V500 cannot be used simultaneously.

Specifications

• Antigen: CD103
• Classification: Monoclonal
• Clone: M290
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 480
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Gene Alias: Itgae, Integrin alpha-E, αE, alpha-E1, ITAE, Integrin αIEL chain, aM290
• Host Species: Rat
• Immunogen: Mouse Intestinal Epithelial Cells
• Purification Method: Affinity Purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ