BV480 CD25 Rat anti-Mouse, Brilliant Violet 480, Clone: PC61, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 566202
The PC61 monoclonal antibody specifically binds to CD25, the low-affinity IL-2 Receptorαchain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rαby itself is not a signaling receptor. However, it can combine with IL-2 Receptorβ(CD122) andγc (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4. The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 and 7D4. It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25.The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436nm and Em Max at 478nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40nm) filter set. BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.
BD Horizon™ BV480 is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436nm and Em Max at 478nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon™ BV510 (525/40nm) filter set. BV480 has less spillover into the BD Horizon™ BV605 detector and, in general, is brighter than BD Horizon BV510. Due to similar excitation and emission properties, BV480, BV510 and V500 cannot be used simultaneously.
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Interleukin-2 receptor alpha chain, IL-2RA, IL-2Rα, Il2ra, IL-2R p55|
|IL-2-dependent cytolytic mouse T-cell clone B6.1|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV480 under optimum conditions, and unconjugated antibody and free BD Horizon BV480 were removed.|
|Brilliant Violet 480|
|Rat OFA, also known as Outbred OFA IgG1, λ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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