Invitrogen™ C. botulinum BoNT-A Light Chain Polyclonal Antibody

Sheep Polyclonal Antibody

Supplier:  Invitrogen™ PA548053

Catalog No. PIPA548053

Description

In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant C. botulinum BoNTA Heavy Chain is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction. E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.

Specifications

• Antigen: C. botulinum BoNT-A Light Chain
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: BONTC1
• Immunogen: E. coli-derived recombinant Clostridium BoNT-A Light Chain Met1-Phe425.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Immunoprecipitation, Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Host Species: Sheep
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov