Invitrogen™ C. botulinum BoNT-A Light Chain Polyclonal Antibody

Catalog No.	Quantity
PIPA548053	100 μg

Catalog No. PIPA548053 Supplier Invitrogen™ Supplier No. PA548053

Description

Sheep Polyclonal Antibody

In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant C. botulinum BoNTA Heavy Chain is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction. E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.

Specifications

• Antigen: C. botulinum BoNT-A Light Chain
• Applications: Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5% trehalose and No Preservative; pH 7.4
• Gene Alias: BONTC1
• Host Species: Sheep
• Immunogen: E. coli-derived recombinant Clostridium BoNT-A Light Chain Met1-Phe425.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Bacteria
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov