c-Jun Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA1833

Catalog No. PA1833

Description

PA1-833 detects cJun from mouse cells. PA1-833 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ∽40 kDa protein representing cJun in NIH 3T3 serum stimulated cells. A smaller band is also detected at ∽26 kDa possibly representing a proteolytic fragment of cJun. The PA1-833 immunogen is a synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun. Reconstitute in 200 μL PBS to create a stock of 1 mg/mL.

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

Specifications

• Antigen: c-Jun
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: JUN
• Gene Alias: Activator protein 1; AH119; AP1; AP-1; Avian sarcoma virus 17 (v-jun) oncogene homolog; cjun; c-Jun; c-jun transcription factor; enhancer-binding protein AP1; I79_009953; immediate early; JUN; Jun A; Jun activation domain binding protein; Jun oncogene; jun proto-oncogene; Jun proto-oncogene, AP-1 transcription factor subunit; Junc; JUND; p39; proto-oncogene c-Jun; Rjg-9; transcription factor; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
• Host Species: Rabbit
• Purification Method: IgG fraction
• Regulatory Status: RUO
• Gene ID (Entrez): 16476
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: ChIP Assay, Western Blot
• Concentration: 1 mg/mL
• Formulation: antibody with 0.05% sodium azide
• Gene Accession No.: P05627
• Gene Symbols: JUN
• Immunogen: Synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG