c-Met Mouse anti-Human, Brilliant Violet 421, Clone: 3D6, BD Optibuild™

Catalog No.	Quantity
BDB742872	50 μg

Catalog No. BDB742872 Supplier BD Biosciences Supplier No. 742872

Description

Mouse Monoclonal Antibody

The 3D6 monoclonal antibody specifically binds to c-Met (MET), which is also known as Hepatocyte growth factor receptor (HGFR) or Scatter factor receptor (SF receptor). c-Met is a 190 kDa single-pass type I transmembrane glycoprotein that is posttranslationally cleaved into a disulfide-linked extracellularα-chain and a transmembraneβ-chain. c-Met functions as a receptor tyrosine kinase (RTK) that is normally involved in the development, regeneration, and survival of cells and tissues. c-Met is expressed on a variety of cell types including stem cells and progenitor cells, hepatocytes, keratinocytes, epithelial cells, endothelial cells, and neurons. c-Met is autophosphorylated when bound by its ligand, Hepatocyte Growth Factor (HGF). This leads to further activation of downstream signaling pathways that induce multiple responses including cellular migration, proliferation, survival, and angiogenesis. Abnormal c-Met expression or activity has been associated with tumorigenesis. The 3D6 antibody can reportedly function as an agonist by binding to and activating human but not mouse c-Met.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: c-Met
• Classification: Monoclonal
• Clone: 3D6
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: MET, cMet, HGFR, HGF receptor, AUTS9, RCCP2, SF receptor
• Host Species: Mouse
• Immunogen: Human c-Met Recombinant Protein
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ