Invitrogen™ c-Mpl Polyclonal Antibody

Catalog No.	Quantity
PIPA547121	100 μg

Catalog No. PIPA547121 Supplier Invitrogen™ Supplier No. PA547121

Description

Goat Polyclonal Antibody

In direct ELISAs, approximately 5% cross-reactivity with recombinant human Tpo R is observed and less than 2% cross-reactivity with recombinant mouse Epo R is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

Specifications

• Antigen: c-Mpl
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5% trehalose and No Preservative
• Gene: MPL
• Gene Accession No.: Q08351
• Gene Alias: CD110; c-mpl; hlb219; MPL; MPL proto-oncogene, thrombopoietin receptor; MPLV; myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene; proto-oncogene c-Mpl; THCYT2; thrombopoietin receptor; TPOR; TPO-R
• Gene Symbols: MPL
• Host Species: Goat
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Thrombopoietin R/Tpo R Ser18-Trp483.
• Purification Method: Antigen Affinity Chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17480
• Target Species: Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov