C-Nap1 (Cep250), Mouse anti-Human, Clone: 150-230-1, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABT1353100UG

Catalog No. MABT1353100

Description

Anti-C-Nap1 (Cep250), clone 150-230-1, Cat. No. MABT1353, is a highly specific mouse monoclonal antibody that targets Centrosome-associated protein CEP250 and has been tested for use in Immunofluorescence and Western Blotting.

Centrosome-associated protein CEP250 (UniProt: Q9BV73; also known as 250 kDa centrosomal protein, Cep250, Centrosomal NEK2-associated protein 1, C-Nap1, Centrosomal protein 2) is encoded by the CEP250 (also known as CEP2, CNAP1) gene (Gene ID: 11190) inn human. Cep250 is a component of the core centrosome that is ubiquitously expressed. It can exist as a monomer and homodimer. It specifically associates with the proximal ends of both mother and daughter centrioles during interphase and in mitotic cells it dissociates from the mitotic spindle poles. At the end of cell division, it is shown to re-accumulate at centrosomes. Cep250 is differentially phosphorylated during cell cycle and phosphorylation status regulates its association/dissociation from centrosome. During M phase its C-terminal part is shown to be phosphorylated by NEK2. Two isoforms of Cep250 have been described that are produced by alternative splicing. Loss of Cep250-mediated centriole cohesion leads to an altered cell migration phenotype.

Specifications

• Antigen: C-Nap1 (Cep250)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: CEP250;CEP2;CNAP1
• Immunogen: Recombinant fragment corresponding to 455 amino acids from the C-terminal region of human Centrosome-associated protein CEP250.
• Quantity: 100 μg
• Research Discipline: Epigenetics & Nuclear Function
• Gene ID (Entrez): NP_001305148
• Target Species: Human
• Form: Purified

• Applications: Immunofluorescence, Western Blot
• Clone: 150-230-1
• Dilution: Immunofluorescence Analysis: A representative lot detected C-Nap1 (Cep250) in Immunofluorescence applications (Fava, L.L., et. al. (2017). Genes Dev. 31(1):34-45).Western Blotting Analysis: A representative lot detected C-Nap1 (Cep250) in HeLa S3 cell lysates. (Courtesy of Prof. Erich A. Nigg, Ph.D., Biozentrum, University of Basel, Switzerland).
• Gene Alias: Centrosome-associated protein CEP250;250 kDa centrosomal protein;Cep250;Centrosomal Nek2-associated protein 1;C-Nap1;Centrosomal protein 2
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 150-230-1 is a mouse monoclonal antibody that detects human Centrosome-associated protein CEP250 (Cep20). It targets an epitope within 455 amino acids from the C-terminal region.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG1 κ