Invitrogen™ c-Raf Monoclonal Antibody (4G4)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA517162

Catalog No. PIMA517162

Description

MA5-17162 targets RAF1 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples. The MA5-17162 immunogen is purified recombinant fragment of human RAF1 expressed in E. Coli.

Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Specifications

• Antigen: c-Raf
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P04049, P11345
• Gene Symbols: Raf1
• Immunogen: Purified recombinant fragment of human RAF1 expressed in E. Coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Clone: 4G4
• Conjugate: Unconjugated
• Gene: Raf1
• Gene Alias: 6430402F14Rik; AA990557; BB129353; CMD1NN; CRAF; C-RAF; C-Raf proto-oncogene, serine/threonine kinase; Craf1; Craf1 transforming; D830050J10Rik; EC 2.7.11.1; kinase Raf1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; NS5; Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; proto-oncogene c-RAF; RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; RAF1; Raf-1; Raf-1 proto-oncogene, serine/threonine kinase; sarcoma 3611 oncogene; v-Raf; v-raf-1 leukemia viral oncogene 1; vraf1 murine le; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; v-raf-leukemia viral oncogene 1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 24703, 5894
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid