Invitrogen™ C. tetani TeNT Light Chain Monoclonal Antibody (604023)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA524273

Catalog No. PIMA524273

Description

In direct ELISAs and Western blots, no cross-reactivity with recombinant TeNT Heavy Chain is observed. Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Tetanus toxin, a member of the peptidase M27 family of proteins, is produced by the anaerobic spore-forming bacteria Clostridium tetani as a single-chain polypeptide that is 1315 amino acids (aa) in length. The protein is subsequently cleaved by an endogenous protease to yield the 52 kDa tetanus toxin light chain (TeNT-LC), which is 457 aa in length, and the 98 kDa tetanus toxin heavy chain that is 858 aa long. The light and heavy chains are linked by a disulfide bridge and are non-toxic after separation. Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neural synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln- -Phe-77 bond of synaptobrevin-2.

Specifications

• Antigen: C. tetani TeNT Light Chain
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunoprecipitation, Western Blot
• Clone: 604023
• Conjugate: Unconjugated
• Gene Alias: TeNT-LC; Tentoxylysin; tetX
• Immunogen: E. coli-derived recombinant C. tetani TeNT Light Chain Pro2-Gly430.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized