MilliporeSigma™ Calbiochem™ Roscovitine

Catalog No. 55-736-05MG

Supplier: MilliporeSigma™ 5573605MG

Description

A potent, reversible, and selective inhibitor of Cdks that exhibits about 10-fold greater efficacy towards p34-cdk1 and p33-cdk2 and 20-fold greater efficacy towards p33-cdk5 relative to Olomoucine.

Inhibits p34cdk1/cyclin B (IC50 = 650 nM), p33cdk2/cyclin A (IC50 = 700 nM), p33cdk2/cyclin E (IC50 = 700 nM), and p33cdk5/p35 (IC50 = 200 nM) by competing for the ATP binding domain of the kinases. Exhibits reduced sensitivity towards related kinases including ERK1 and ERK2 (IC50 = 34μM and 14μM, respectively). Roscovitine does not significantly affect the activity of other protein kinases even at 100μM, including p34cdk4/cyclin D1 and p40cdk6/cyclin D2. Compared to olomoucine, roscovitine displays increased anti-mitotic activity at the G1/S and G2/M phases of the cell cycle. A 50mm (5mg/282μl) solution of Roscovitine (Cat. No. 557364) in DMSO is also available.

Specifications

• Color: White to Off-white
• Target Proteins: p34cdk1/cyclin B
• CAS: 186692-46-6
• Molecular Formula: C₁₉H₂₆N₆O
• Formula Weight: 354.5g/mol
• Solubility: DMSO
• Form: Solid
• Quantity: 5 mg
• Purity: 95% by HPLC