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Invitrogen™ eBioscience™ Calcein AM Viability Dye (UltraPure Grade)

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Quantity:
1 mg
20 x 50 μg
Description
- Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye
- Apoptotic and dead cells with compromised cell membranes do not retain Calcein
- Calcein is optimally excited at 495 nm and has a peak emission of 515 nm
- For flow cytometric analysis, it can be excited off the blue laser line (488 nm) and detected using filters for FITC/Alexa Fluor™ 488 (530/30)
- For multicolor flow cytometry, concentrations in the range of 5 to 20 nM are recommended to ensure that compensation can be performed successfully
- Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
- If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854)
- Calcein AM should be reconstituted in high-quality, freshly opened DMSO
- Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time (avoid freeze-thawing)
- Purity: >95% (determined by HPLC)

Specifications
Specifications
Color | Green |
Detection Method | Fluorescence |
For Use With (Application) | Viability Assay |
For Use With (Equipment) | Fluorescence Microscope, Flow Cytometer |
Product Type | Dye |
Emission | 495/515 |
Product Line | eBioscience |
Quantity | 20 x 50 μg |
Safety and Handling
WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
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