Invitrogen™ eBioscience™ Calcein Blue AM Viability Dye

Description

Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Blue.

• Molecular weight: 465.41g/mol
• Peak excitation: 360nm
• Peak emission: 445nm
• Reconstitute in high-quality, freshly opened DMSO
• Once reconstituted, store at −20°C with dessicant and use within a short period of time. Avoid freeze-thawing

• Calcein Blue is optimally excited by the UV laser line (360nm), but can also be excited by the violet laser line (405nm)
• For fluorescent microscopy, detect using the appropriate filter sets
• Calcein Blue AM is itself weakly fluorescent, and an additional wash step may be necessary to reduce background fluorescence
• Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells

Specifications

• Color: Blue
• Detection Method: Fluorescence
• For Use With (Application): Viability Assay
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Product Type: Dye
• Emission: 408/450
• Product Line: eBioscience
• Quantity: 1 mg

For Research Use Only. Not for use in diagnostic procedures.