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Invitrogen™ eBioscience™ Calcein Violet 450 AM Viability Dye

Catalog No. 5016954
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Quantity:
1 mg

Description

Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450.

  • Molecular weight: 600g/mol
  • Peak excitation: 408nm
  • Peak emission: 450nm
  • Reconstitute in high-quality, freshly opened DMSO
  • Once reconstituted, store at −20°C with dessicant and use within a short period of time. Avoid freeze-thawing
  • For fluorescent microscopy, detect using the appropriate filter sets
  • For flow cytometric analysis, excite with the violet laser line (405nm) and detect using filters for Pacific Blue™/eFluor™ 450 (450/50)
  • Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
TRUSTED_SUSTAINABILITY

Specifications

Color Violet
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Dye
Emission 408/450
Product Line eBioscience
Quantity 1 mg

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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