Invitrogen™ Calcineurin B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529939

Catalog No. PIPA529939

Description

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse fetal brain, rat brain, MEF. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (97%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin (also referred to as protein phosphatase 2B) is composed of two subunits; calcineurin A (CnA), a 60 kDa catalytic subunit which is highly homologous to protein phosphatases 1 and 2A; and a 19 kDa regulatory subunit, calcineurin B (CnB) which, like calmodulin, contains four EF-hand calcium binding sites. Calcineurin-mediated dephosphorylation of the nuclear factor of activated T-cells (NF-AT) is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. The immunosuppressive drugs, cyclosporin A and FK506, when bound to the immunophilins, cyclophilin and FKBP respectively, inhibit dephosphorylation and activation of NF-AT by calcineurin. This inhibition is caused by the immunosuppressant/immunophilin complex simultaneously binding to both CnA and CnB which does not inhibit the phosphatase activity of CnA, but rather blocks access of the substrate to the catalytic domain of CnA.

Specifications

• Antigen: Calcineurin B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PPP3R1
• Gene Alias: ALNB1; calcineurin B, type I (19kDa); calcineurin subunit B type 1; CALNB1; CaNB1; Cna2; Cnb; Cnb1; MCIP1; PP2B beta 1; PPP3R1; protein phospatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I); protein phospatase 3, regulatory subunit B, alpha isoform,type 1; protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; protein phosphatase 3 regulatory subunit B alpha; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3 regulatory subunit B, alpha; protein phosphatase 3, regulatory subunit B, alpha; protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19058, 29748, 5534
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.76 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P63098, P63100, Q63810
• Gene Symbols: PPP3R1
• Immunogen: Recombinant protein fragment corresponding to a region within amino acids 1 and 154 of Human Calcineurin B.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov