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  6. Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514 

Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514

Catalog No. 5016960
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10 x 50μg
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50-169-60 10 x 50μg
50-169-59 1mg
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Catalog No. 50-169-60 Supplier Invitrogen™ Supplier No. 65085970
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Description

Membrane-permeable dye used to monitor changes in intracellular free calcium concentrations in the cell. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ≈520nm.

  • Calcium binding affinity: Kd = 232 nM
  • Molecular Weight: ≈1100 d
  • Peak Excitation: 490nm
  • Peak Emission: 514nm
  • Reconstitute in high-quality, freshly opened DMSO
  • Recommended concentration: 2 to 5 mM
  • Once reconstituted, protect from light and store dessicated at −20°C; avoid freeze-thawing
TRUSTED_SUSTAINABILITY

Specifications

Detection Method Fluorescence
For Use With (Application) Calcium Assay
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Product Type Dye
Emission 490/514
Product Line eBioscience
Quantity 10 x 50μg

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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