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Caldesmon/CALD1 Antibody (h-CALD), Novus Biologicals™
SDP

Catalog No. p-5539588 Shop All R&D Systems Products
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NBP24442100 0.02 mg
NBP24442101 0.1 mg
NBP24442102 0.2 mg
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Catalog No. NBP24442100 Supplier Novus Biologicals Supplier No. NBP2444210.02MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Simple Western, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Caldesmon/CALD1 Monoclonal specifically detects Caldesmon/CALD1 in Human samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

Antigen Caldesmon/CALD1
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Classification Monoclonal
Clone h-CALD
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Simple Western 10 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. Q05682
Gene Alias CAD, caldesmon, caldesmon 1, CDMH-CAD, HCAD, LCAD, L-CAD, MGC21352, NAG22
Gene Symbols CALD1
Host Species Mouse
Immunogen Crude human uterus extract
Molecular Weight of Antigen 150 kDa
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Cytoskeleton Markers
Primary or Secondary Primary
Gene ID (Entrez) 800
Test Specificity Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Target Species Human
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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