Invitrogen™ Calnexin Monoclonal Antibody (3H4A7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA515389

Catalog No. PIMA515389

Description

MA5-15389 targets Calnexin in indirect ELISA, IF and WB applications and shows reactivity with Human samples. The MA5-15389 immunogen is synthetic peptide corresponding to aa (CEAAEERPWLWVVYILTVAL) of human Calnexin, conjugated to KLH. MA5-15389 detects Calnexin which has a predicted molecular weight of approximately 90kDa.

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

Specifications

• Antigen: Calnexin
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: ascites with 0.03% sodium azide
• Gene Accession No.: P27824, P35564
• Gene Symbols: CANX
• Immunogen: Synthetic peptide corresponding to aa (CEAAEERPWLWVVYILTVAL) of human Calnexin, conjugated to KLH (aa 475-493).
• Regulatory Status: RUO
• Gene ID (Entrez): 12330, 821
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Clone: 3H4A7
• Conjugate: Unconjugated
• Gene: CANX
• Gene Alias: 1110069N15Rik; AI988026; calmegin; calnexin; canx; canx-a; canx-b; clgn; clnx; cnx; D11Ertd153e; FLJ26570; hypothetical protein; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; P90; PP90; pp90 precursor
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2b