Invitrogen™ Calnexin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA534804

Catalog No. PIPA534804

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse liver, PC-12, Rat2. Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (82%), Xenopus laevis (86%), Dog (98%), Pig (96%), Chicken (92%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

Specifications

• Antigen: Calnexin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CANX
• Gene Alias: 1110069N15Rik; AI988026; calmegin; calnexin; canx; canx-a; canx-b; clgn; clnx; cnx; D11Ertd153e; FLJ26570; hypothetical protein; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; P90; PP90; pp90 precursor
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12330, 29144, 821
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.53 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P27824, P35564, P35565
• Gene Symbols: CANX
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov