Invitrogen™ Calnexin Polyclonal Antibody

Catalog No.	Quantity
PIPA577839	200 μg

Catalog No. PIPA577839 Supplier Invitrogen™ Supplier No. PA577839

Description

Rabbit Polyclonal Antibody

A 1:2,000 dilution of PA5-77839 was sufficient for detection of Calnexin in 10 μg of HeLa cell lysate by ECL immunoblot analysis. Detects the C-terminal domain of Calnexin approximately 90kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

Specifications

• Antigen: Calnexin
• Applications: Flow Cytometry, Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.2
• Gene: CANX
• Gene Accession No.: P00789, P24643, P27824, P35564, P35565
• Gene Alias: 1110069N15Rik; AI988026; calmegin; calnexin; canx; canx-a; canx-b; clgn; clnx; cnx; D11Ertd153e; FLJ26570; hypothetical protein; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; P90; PP90; pp90 precursor
• Gene Symbols: CANX
• Host Species: Rabbit
• Immunogen: Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
• Purification Method: Antigen affinity chromatography, Protein A
• Quantity: 200 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100341645, 100519184, 100689345, 100726237, 101122273, 101828013, 105217515, 107320193, 12330, 29144, 403908, 407129, 416288, 448161, 462330, 821
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Bovine, Sheep, Hamster, Pig, Chicken, Drosophila, Guinea Pig, Avian, Frog, Chimpanzee
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG