Calponin 1 Antibody (CNN1/832 + CALP) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2477580.1MG

Catalog No. NBP247758A

Description

Calponin 1 Monoclonal specifically detects Calponin 1 in Human, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: Calponin 1
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Gene Alias: Basic calponin, calponin 1, basic, smooth muscle, Calponin H1, smooth muscle, calponins, basic, Sm-Calp, SMCCcalponin-1
• Host Species: Mouse
• Molecular Weight of Antigen: 34 kDa
• Quantity: 0.1 mg
• Research Discipline: Cancer, Cytoskeleton Markers
• Gene ID (Entrez): 1264
• Target Species: Human, Rat
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: CNN1/832 + CALP
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: CNN1
• Immunogen: Recombinant full-length human CNN1 protein (CNN1/832); Crude human uterus extract (CALP)
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG