Calretinin, Rabbit anti-Rat, Human, Polyclonal, Millipore Sigma™

Catalog No.	Quantity
ABN219125UL	25 μL

Catalog No. ABN219125UL Supplier MilliporeSigma Supplier No. ABN219125UL

Description

Rabbit Polyclonal Antibody

Anti-Calretinin Antibody, Cat. No. ABN2191, is a highly specific rabbit polyclonal antibody that targets Calretinin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Calretinin (UniProt: P47728; also known as CR) is encoded by the Calb2 gene (Gene ID: 117059) in rat. Calretinin belongs to the calbindin protein family and acts as a calcium-binding protein. It is expressed in both central and peripheral nervous system and is found abundantly in auditory neurons. It is involved in regulating cytosolic calcium ion concentrations and has multiple calcium-binding domains known as EF-hands. Its expression is shown to be regulated at the level of transcription by nuclear respiratory factor 1 (NRF-1) and E2F2 transcription factors. In rat striatum calretinin neurons form a distinct subset of medium-sized (7 20μm), aspiny, GABAergic interneurons. Calretinin can also serve as an important differential diagnostic tool for cystic and solid ameloblastic tumors. (Ref.: Petryszyn, S., et al. (2017). J. Neural Transm. doi: 10.1007/s00702-017-1687; Kresoja-Rakic, J., et al. (2017). Front. Genet. doi: 10.3389/fgene.2017.00070).

Specifications

• Antigen: Calretinin
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: A 1:500 dilution from a representative lot detected Calretinin in rat brain tissue lysate.Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Calretinin in human cerebral cortex and rat cerebellum tissue sections.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: CR
• Gene Symbols: Calb2
• Host Species: Rabbit
• Immunogen: GST/His-tagged recombinant fragment corresponding to 99 amino acids from the internal region of rat Calretinin.
• Molecular Weight of Antigen: ∽31 kDa observed; 31.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Purification Method: Affinity Purified
• Quantity: 25 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody detects Calretinin in human and rat cells. It targets an epitope within 99 amino acids from the internal region.
• Target Species: Rat, Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG