Invitrogen™ CAMSAP3 Polyclonal Antibody

Catalog No.	Quantity
PA548993	400 μL

Catalog No. PA548993 Supplier Invitrogen™ Supplier No. PA548993

Description

Rabbit Polyclonal Antibody

CAMSAP3 Polyclonal Antibody for Western Blot

Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:19041755, PubMed:23169647). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24486153). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153). In addition, it also reduces the velocity of microtubule polymerization (PubMed:24486153). Required for the biogenesis and the maintenance of zonula adherens by anchoring the minus-end of microtubules to zonula adherens and by recruiting the kinesin KIFC3 to those junctional sites (PubMed:19041755). Required for orienting the apical-to-basal polarity of microtubules in epithelial cells: acts by tethering non-centrosomal microtubules to the apical cortex, leading to their longitudinal orientation (PubMed:27802168, PubMed:26715742). Plays a key role in early embryos, which lack centrosomes: accumulates at the microtubule bridges that connect pairs of cells and enables the formation of a non-centrosomal microtubule-organizing center that directs intracellular transport in the early embryo. Couples non-centrosomal microtubules with actin: interaction with MACF1 at the minus ends of non-centrosomal microtubules, tethers the microtubules to actin filaments, regulating focal adhesion size and cell migration (PubMed:27693509). Plays a key role in the generation of non-centrosomal microtubules by accumulating in the pericentrosomal region and cooperating with KATNA1 to release non-centrosomal microtubules from the centrosome (PubMed:28386021). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed:28089391). Through interaction with AKAP9, involved in translocation of Golgi vesicles in epithelial cells, where microtubules are mainly non-centrosomal (PubMed:28089391). [UniProt].

Specifications

• Antigen: CAMSAP3
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: 2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene: CAMSAP3
• Gene Accession No.: Q80VC9, Q9P1Y5
• Gene Alias: 2310057J16Rik; calmodulin regulated spectrin associated protein family member 3; calmodulin regulated spectrin-associated protein family, member 3; calmodulin-regulated spectrin-associated protein 3; CAMSAP3; KIAA1543; Marshalin; marshalin-La; marshalin-Lb; marshalin-Lc; marshalin-Ld; marshalin-Sa; marshalin-Sb; marshalin-Sc; marshalin-Sd; Nezha; PPP1R80; Protein Nezha; protein phosphatase 1, regulatory subunit 80
• Gene Symbols: CAMSAP3
• Host Species: Rabbit
• Immunogen: KLH conjugated synthetic peptide between 103-131 amino acids from the N-terminal region of human CAMSAP3.
• Purification Method: Antigen affinity chromatography, Protein A
• Quantity: 400 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 57662, 69697
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG