R&D Systems™ Recombinant Canine FGF basic/FGF2/bFGF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Canine FGF basic/FGF2 Protein is derived from E. coli. The Recombinant Canine FGF basic/FGF2 Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: FIP6K6
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in MOPS, Na 2 SO 4 TCEP and EDTA with BSA as a carrier protein.
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): Observed M.W.: 17kDa, reducing conditions, Theoretical M.W.: 16kDa
• Quantity: 25 μg
• Source: E. coli-derived canine FGF basic/FGF2/bFGF protein Pro10-Ser155 (Lys36Asn)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
• Common Name: FGF basic/FGF2
• Gene Symbol: FGF2
• Biological Activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 0.2-1ng/mL.
• Conjugate: Unconjugated
• Species: Canine
• Recombinant: Recombinant
• Expression System: E. coli-derived Pro10-Ser155 (Lys36Asn)
• Purity or Quality Grade: >95%, by SDS-PAGE with silver staining.