CAPS, 0.5 M Buffer Solution, pH 10.5, PROMO, Thermo Scientific Chemicals

Description

Applications

CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI>8.5. CAPS is not one of the original Good's buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.

Solubility

Soluble in water (100 g/L) at 20°C, and methanol (1%).

Notes

Store at room temperature. Ensure good ventilation. Incompatible with oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 1135-40-6
• Molecular Formula: C9H19NO3S
• Molecular Weight (g/mol): 221.32 g/mol
• MDL Number: MFCD00003837
• InChI Key: PJWWRFATQTVXHA-UHFFFAOYSA-N
• IUPAC Name: 3-(cyclohexylamino)propane-1-sulfonic acid
• SMILES: OS(=O)(=O)CCCNC1CCCCC1

• Concentration: 0.5 M
• pH: 10.5
• Physical Form: Liquid
• Quantity: 500 mL
• Chemical Name or Material: CAPS

RUO - Research Use Only