Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. 5943752200

Thermo Scientific™ CaptureSelect™ LambdaXP RoboColumn™ Pre-packed columns

Catalog No. 5943752200
Click to view available options
Quantity:
1 Strip (8 Pieces), 200 μL

Description

Thermo Scientific™ CaptureSelect™ RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

The CaptureSelect LambdaXP affinity matrix in this RoboColumn recognizes the constant domain of the human lambda light chain enabling purification of all IgG isotypes, including IgG, IgA, IgM, IgD, and IgE, directly from complex source materials in a robust and efficient purification process with excellent purity obtained in a single step.

Features of the LambdaXP affinity matrix include:

  • Efficient elution of Fab fragments and immunoglobulins containing a lambda light chain; no cross binding to the Fc-region or kappa light chains
  • One-step purification from crude material with high purity and yield
  • Human specific
  • Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

  • Column size: 200 μL, 1 strip, 8 columns (8-row)
  • Column dimension: 5-mm diameter, 10-mm bed height
  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand:CaptureSelect LambdaXP affinity ligand
  • Ligand coupling: epoxide coupling
  • Binding capacity: >30 g IgG/L resin depending on flow rate, column height, and contact time
  • Elution conditions: 25 mM sodium acetate, pH 3.5
  • Formulation buffer: 20% (v/v) ethanol
  • Operating pressure: 2 bar (maximum)
  • Flow rate: 16–150 cm/hr

RoboColumn is a trademark of Repligen.

Specifications

Capacity (Metric) 200 μL
Particle Size 65 ±10 μm
Product Line CaptureSelect™
Quantity 1 Strip (8 Pieces), 200 μL
Format Robo Column
Column Type Affinity, Prepacked
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.