Carbonic Anhydrase I Polyclonal Antibody, Biotin, Rockland™

Goat Polyclonal Antibody

Supplier:  Rockland Immunochemicals 20016540100

Catalog No. 50-199-0480

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-CARBONIC ANHYDRASE I (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase I from other sources may occur but has not been specifically determined.

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.

Specifications

• Antigen: Carbonic Anhydrase I
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: CA1
• Gene Alias: AW555628; CA I; CA1; ca1 {ECO:0000250; CAB; CA-I; CA-I {ECO:0000250; Car1; Car-1; Carbonate dehydratase I; carbonate dehydratase I {ECO:0000250; Carbonic anhydrase 1; carbonic anhydrase 1 {ECO:0000250; Carbonic anhydrase B; Carbonic anhydrase I; carbonic anhydrase I {ECO:0000250; epididymis secretory protein Li 11; HEL-S-11; UniProtKB:P00915}
• Host Species: Goat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P00915
• Gene Symbols: CA1
• Immunogen: Carbonic Anhydrase I [Human Erythrocytes]
• Regulatory Status: RUO
• Gene ID (Entrez): 759
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized