carboxypeptidase M, Mouse anti-Rat, Clone: 7F9, Millipore Sigma™

Catalog No.	Quantity
MABS192525	25 μg

Catalog No. MABS192525 Supplier MilliporeSigma Supplier No. MABS192525UG

Description

Mouse Monoclonal Antibody

Anti-carboxypeptidase M, clone 7F9, Cat. No. MABS1925, is a mouse monoclonal antibody that detects Carboxypeptidase M and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Carboxypeptidase M (UniProt: D4A9Q5; also known as CPM) is encoded by the Cpm gene (Gene ID: 314855) in rat. Rat CPM a glycosylphosphatidylinositol (GPI) anchored metallo-carboxypeptidase that cleaves basic carboxyl-terminal amino acids from various peptides at neutral pH. Rat CPM is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form. The mature CPM displays high specificity and cleaves only C-terminal arginine or lysine residues in a variety of peptide substrates. It is shown to be expressed in the lung and placenta at high levels. Its expression is detected in alveolar cells lacking T1alpha, a type I cell marker protein found in adult lung. Carboxypeptidase M also serves as a macrophage differentiation marker, which is preferentially induced in epithelioid cells of all granuloma types studied, but not in resting macrophages. Hence, it can be employed for detection of minute granuloma even in the presence of non-granulomatous macrophages. Clone 7F9 recognizes CPM expressed in late embryonic rat lung to postnatal day 1. (Ref.: Fujiwara, N., et al. (2007), Respirology 12(1); 54-62; Tsakiris, I., et al. (2012). Lab. Invest. 92(3); 345-361).

Specifications

• Antigen: carboxypeptidase M
• Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 7F9
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: A representative lot detected carboxypeptidase M in Western Blotting applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunoprecipitation Analysis: A representative lot immunoprecipitated carboxypeptidase M in Immunoprecipitation applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).Immunohistochemistry Analysis: A representative lot detected carboxypeptidase M in Immunohistochemistry applications (Fujiwara, N., et. al. (2007). Respirology. 12(1):54-62).
• Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: CPM;CP-M
• Gene Symbols: Cpm
• Host Species: Mouse
• Immunogen: Membrane fraction of E18.5 embryonic rat lung.
• Purification Method: Protein G purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001101568.1
• Test Specificity: Clone 7F9 specifically detects carboxypeptidase M in rat tissues.
• Target Species: Rat
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2b κ