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MilliporeSigma™ Cas9, CT, Mouse, Unlabeled, Clone: 10C11-A12,

Catalog No. MABS1291MI Shop All MilliporeSigma Products
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Quantity:
100 μg
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
MABS1291MI 100 μg Unconjugated
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Catalog No. MABS1291MI Supplier MilliporeSigma™ Supplier No. MABS1291
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Description

Mouse Monoclonal Antibody

CRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1's CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3&prime-5&prime exonucleolytically.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Cas9, CT
Applications Immunoprecipitation, Western Blot
Classification Monoclonal
Clone 10C11-A12
Concentration Please refer to lot specific datasheet.
Conjugate Unconjugated
Formulation Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene cas9, csn1
Gene Accession No. Q99ZW2
Host Species Mouse
Immunogen His-tagged recombinant S.pyogenes Cas9 C-terminal fragment.
Purification Method Protein G Purified
Quantity 100 μg
Regulatory Status RUO
Research Discipline Signaling
Primary or Secondary Primary
Gene ID (Entrez) NP_269215
Test Specificity Clone 10C11-A12 recognizes an epitope located in the C-terminal region of Cas9.
Target Species Bacteria
Content And Storage Stable for 1 year at 2°-8°C from date of receipt.
Form Purified
Isotype IgG1 κ
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For Research Use Only. Not for use in diagnostic procedures.

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