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MilliporeSigma™ Cas9, CT, Mouse, Unlabeled, Clone: 6G12-H11,

Catalog No. MABE1668MI Shop All MilliporeSigma Products
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Quantity:
100 μg
Conjugate:
Unconjugated
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Catalog No. Quantity Conjugate
MABE1668MI 100 μg Unconjugated
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Catalog No. MABE1668MI Supplier MilliporeSigma™ Supplier No. MABE1668
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Description

Mouse Monoclonal Antibody

CRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene Cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1's CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3&prime-5&prime exonucleolytically.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Cas9, CT
Applications Western Blot
Classification Monoclonal
Clone 6G12-H11
Concentration Please refer to lot specific datasheet.
Conjugate Unconjugated
Formulation Purified mouse IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene cas9, csn1
Gene Accession No. Q99ZW2
Host Species Mouse
Immunogen His-tagged recombinant Cas9 C-terminal fragment excluding the active sites RuvC-II & RuvC-III.
Purification Method Protein G Purified
Quantity 100 μg
Regulatory Status RUO
Research Discipline Epigenetics & Nuclear Function
Primary or Secondary Primary
Gene ID (Entrez) NP_269215
Test Specificity Clone 6G12-H11 targets an epitope within Cas9 C-terminal region outside the active sites RuvC-II & RuvC-III.
Target Species All species
Content And Storage Stable for 1 year at 2°-8°C from date of receipt.
Form Purified
Isotype IgG1 κ
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For Research Use Only. Not for use in diagnostic procedures.

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