MilliporeSigma™ Cas9, D10A/H840A Mutant, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABC1490

Catalog No. ABC1490MI

Description

CRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene Cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1's CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3&prime-5&prime exonucleolytically. Mutations of the catalytic residues (D10A in RuvC and H840A in HNH) convert Cas9 into DNA nickases (Cas9n) that cleave only the strand complementary to the crRNA, minimizing off-target activity.

Specifications

• Antigen: Cas9, D10A/H840A Mutant
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: cas9, csn1
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This polyclonal antibody will detect Cas9 with D10A mutation in the RuvC-I region and/or H840A mutation in the HNH domain (e.g. AKA60242.1), but not Cas9 with neither D10A nor H840A mutation (e.g. NP_269215).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

• Applications: Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: Q99ZW2
• Immunogen: A mixture of two KLH-conjugated peptides corresponding to the Cas9 target sites sequences with D10A and H840A mutations.
• Quantity: 100 μg
• Research Discipline: Secondary & Control Antibodies
• Gene ID (Entrez): AKA60242.1
• Target Species: All species

For Research Use Only. Not for use in diagnostic procedures.