Invitrogen™ Cas9 Monoclonal Antibody (3F9)

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA547317	Invitrogen™ MA547317	500 μL
PIMA547415	Invitrogen™ MA547415	100 μL

Description

Western blot dilution: 1:1,000-1:2,000 (CAS9 transfected cells); 1:10,000-20,000 (pure full length CAS9 protein).

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Specifications

• Antigen: Cas9
• Concentration: 1 mg/mL
• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 50% glycerol and 5mM sodium azide
• Immunogen: N-terminal region, amino acids 1-608 of Cas9 sequence CDJ55032.1 from Streptococcus pyogenes, expressed in and purified from E. coli.
• Product Type: Antibody

• Clone: 3F9
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Bacteria
• Gene Alias: cas9; CRISPR9; CRISPR-Cas9; s aureus
• Primary or Secondary: Primary
• Purification Method: Protein A/G