Invitrogen™ Cas9 Monoclonal Antibody (8-A6-6)

Catalog No.	Quantity
PIMA545043	100 μL

Catalog No. PIMA545043 Supplier Invitrogen™ Supplier No. MA545043

Description

Mouse Monoclonal Antibody

Cas9 Monoclonal Antibody for Western Blot, ICC/IF

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Specifications

• Antigen: Cas9
• Applications: Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 8-A6-6
• Concentration: 2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.2% BSA, 50% glycerol and 0.05% sodium azide; pH 7.4
• Gene Alias: cas9; CRISPR9; CRISPR-Cas9; s aureus
• Host Species: Mouse
• Immunogen: Recombinant protein within Streptococcus Pyogenes CRISPR-Cas9 aa 1-200.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Bacteria
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgM