Active Motif Cas9 Monoclonal Antibody (8C1-F10), Active Motif™

Catalog No.	Quantity
50-199-3675	100 μg
50-199-3676	10 μg
50-227-7258	50 μg

Catalog No. 50-199-3675 Supplier Active Motif Supplier No. 61757

Description

Mouse Monoclonal Antibody

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Specifications

• Antigen: Cas9
• Applications: ChIP Assay, Immunoprecipitation, Western Blot, Immunocytochemistry, Western Blot
• Classification: Monoclonal
• Clone: 8C1-F10
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
• Gene: CASP9
• Gene Accession No.: P55211, Q8C3Q9
• Gene Alias: cas9; CRISPR9; CRISPR-Cas9; s aureus
• Gene Symbols: CASP9
• Host Species: Mouse
• Immunogen: This antibody was raised against a recombinant protein within the N-terminal region of <i>Streptococcus pyogene</i> Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12371, 842
• Target Species: Bacteria, Human, Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b