Invitrogen™ Caspase 1 Monoclonal Antibody (14F468)

Catalog No.	Quantity
PIMA516215	100 μg

Catalog No. PIMA516215 Supplier Invitrogen™ Supplier No. MA516215

Description

Mouse Monoclonal Antibody

Suggested positive control: 3T3 whole cell lysate, antigen standard for CASP1 (transient overexpression lysate), HeLa or NIH-3T3, Hela whole cell lysate. Immunogen's sequence similarity with other species: Porcine/Pig (85%), Equine/Horse (80%), Canine (70%). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

Specifications

• Antigen: Caspase 1
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 14F468
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% sodium azide
• Gene: CASP1
• Gene Accession No.: P29452, P29466, P43527
• Gene Alias: CASP1; CASP-1; CASP1 nirs variant 1; caspase 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase1; caspase-1; Caspase-1 subunit p10; Caspase-1 subunit p20; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL1BC; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
• Gene Symbols: CASP1
• Host Species: Mouse
• Immunogen: Two synthetic peptides from the human Caspase-1 protein (amino acids 371-390 and 31-45).
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12362, 25166, 834
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ