Invitrogen™ Caspase 1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
01-673-157	Invitrogen™ PA137232	1 mL
PIPA532292	Invitrogen™ PA532292	500 μL

Description

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta tissue.

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.

Specifications

• Antigen: Caspase 1
• Applications: Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: P29466
• Gene ID (Entrez): 834
• Immunogen: Synthetic peptide derived from internal region of human caspase 1 protein (aa 200-404).
• Product Type: Antibody

• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Classification: Polyclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human
• Gene: CASP1
• Gene Alias: CASP1; CASP-1; CASP1 nirs variant 1; caspase 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase1; caspase-1; Caspase-1 subunit p10; Caspase-1 subunit p20; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL1BC; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
• Gene Symbols: CASP1
• Primary or Secondary: Primary
• Purification Method: Antigen affinity chromatography