Caspase-3 Antibody (31A1067) - (Pro and Active) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP233244

Catalog No. NBP233244

Description

Caspase-3 Monoclonal specifically detects Caspase-3 in Human, Mouse, Rat, Porcine, Chicken, Chinese Hamster, Mammal samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Knockout Validated, Knockdown Validated.

Specifications

• Antigen: Caspase-3
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Dilution: Western Blot 1-5 ug/ml, Simple Western, Flow Cytometry, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 23840553), Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (Lee et al), Immunohistochemistry-Frozen 1:10-1:500. Use reported in scientific literature (Zhang et al), Knockout Validated, Knockdown Validated
• Gene Alias: Apopain, apoptosis-related cysteine protease, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
• Host Species: Mouse
• Molecular Weight of Antigen: 31.7 kDa
• Quantity: 0.1 mg
• Research Discipline: Apoptosis, Cancer, Caspases, Phospho Specific
• Gene ID (Entrez): 836
• Target Species: Human, Mouse, Rat, Pig, Chicken, Chinese Hamster, Mammalia
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoassay, KnockDown
• Clone: 31A1067
• Conjugate: Unconjugated
• Gene Accession No.: P42574
• Gene Symbols: CASP3
• Immunogen: This Caspase-3 Antibody (31A1067) - (Pro and Active) - Azide and BSA Free was developed against full-length recombinant human caspase-3 protein.
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The antibody detects both pro Caspase-3 (∼32 kDa) and the large subunit of the active/cleaved form (∼14-21 kDa) of Caspase-3.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only