Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 155 publications

Catalog Number	Mfr. No.	Quantity
NB10056708T	Novus Biologicals NB10056708SS	0.025 mg
NB10056708	Novus Biologicals NB10056708	0.1 mg

Description

Caspase-3 Monoclonal specifically detects Caspase-3 in Human, Mouse, Rat, Porcine, Chicken, Chinese Hamster, Mammal samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Hematoxylin & Eosin Stain, Electron Microscopy, Immunohistochemistry Free-Floating, CyTOF-ready, Knockout Validated, Knockdown Validated.

Specifications

• Antigen: Caspase-3
• Concentration: 1 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot, Immunoassay, Immunohistochemistry (Free Floating), CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Cancer, Caspases, Phospho Specific
• Gene Accession No.: P42574
• Gene ID (Entrez): 836
• Immunogen: This Caspase-3 Antibody (31A1067) - (Pro and Active) was developed against full-length recombinant human caspase-3 protein.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 31.7 kDa

• Clone: 31A1067
• Dilution: Western Blot 1 - 5 ug/ml, Simple Western 1:50, Flow Cytometry reported in scientific literature (PMID 27429862), Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 23840553), Immunohistochemistry-Paraffin 1:10 - 1:500. Use reported in scientific literature (PMID 28500555), Immunohistochemistry-Frozen 1:10 - 1:500. Use reported by customer review, Immunoblotting reported in scientific literature (PMID 28500555), Hematoxylin and Eosin Stain reported in scientific literature (PMID 28186963), Electron Microscopy reported in scientific literature (PMID 27450722), Immunohistochemistry Free-Floating reported in scientific literature (PMID 31771656), CyTOF-ready, Knockout Validated, KnockDown Validated reported in scientific literature (PMID 32867814)
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Chinese Hamster, Mammalia
• Gene Alias: Apopain, apoptosis-related cysteine protease, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
• Gene Symbols: CASP3
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Test Specificity: The antibody detects both pro Caspase-3 (∼32 kDa) and the large subunit of the active/cleaved form (∼14-21 kDa) of Caspase-3.

For Research Use Only