Invitrogen™ Caspase 3 p17 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA578921

Catalog No. PIPA578921

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: Rat Heart Tissue, Rat Liver Tissue, Rat Thymus Tissue, Rat Spleen Tissue, mouse thymus tissue, mouse spleen tissue, mouse lung tissue, mouse brain tissue, mouse testis tissue. IHC: rat intestine tissue.

CASP3 (Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9. It is encoded by the CASP3 gene. The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family. Tiso et al. (1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al. (1994) found that overexpression of CPP32 in insect cells induced apoptosis. Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis. Tewari et al. (1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.

Specifications

• Antigen: Caspase 3 p17
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CASP3
• Gene Alias: A830040C14Rik; AC-3; Apopain; Casp3; CASP-3; caspase 3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; CC3; Cleaved Caspase 3; Cpp32; CPP-32; CPP32beta; cysteine protease CPP32; IRP; LICE; mldy; protein Yama; SCA-1; SREBP cleavage activity 1; Yama; yama protein
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12367, 25402
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide, 0.05mg thimerosal
• Gene Accession No.: P55213, P70677
• Gene Symbols: CASP3
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse Caspase-3 (P17) (67-81aa TDVDAANLRETFMGL).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov